Part:BBa_J70012:Design

A BioScaffold Part (Uses PsrI), Protein Head Remover see Part Design page

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 25
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Can be used to removed protein heads for protein fusions, see BBF RFC15

TACTAGAG and TACAGAT represent sequences for BioBrick scars that are created when

two BioBrick parts are fused together.

^s represent restriction enzyme cut sites.

General Function

XXXXX XXXXX^TACTAGAG  BioScaffold Part BBa_J70012 TACTAGAT XX XXXXX^XXXXX
XXXXX^XXXXX ATGATCAC                              ATGATCTA XX^XXXXX XXXXX

Function when the right part is a protein part.

XXXXX XXXXX^TACTAGAG  BioScaffold Part BBa_J70012 TACTAGAT GX XXXXX^XXXXX
XXXXX^XXXXX ATGATCAC                              ATGATCTA CX^XXXXX XXXXX

What happens after cutting with MabI or AscI and purifying with either gel purification

or a Qiagen PCR cleanup spin column

General Case

XXXXX XXXXX^                                                    ^XXXXX
XXXXX^                                                    ^XXXXX XXXXX

Case when right part is a protein part

XXXXX XXXXX^                                                    ^XXXXX
XXXXX^                                                    ^XXXXX XXXXX

Source

designed